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Image Search Results
Journal: Clinical and Translational Medicine
Article Title: Milk fat globule—EGF factor 8/ATP‐binding cassette subfamily E member 1 axis maintains mitophagy flux homeostasis to suppress ferroptosis in acute pancreatitis
doi: 10.1002/ctm2.70619
Figure Lengend Snippet: Inhibition of ferroptosis antagonised the aggravating effect of milk fat globule—epidermal growth factor 8 knockout (MFG‐E8‐KO) on experimental acute pancreatitis (AP). (A) Representative images of haematoxylin and eosin (H&E) staining of the pancreas (200×). (B) Pancreatic injury scores. (C) Serum amylase levels. (D) Serum lactate dehydrogenase (LDH) levels. (E) Serum tumour necrosis factor‐alpha (TNF‐α) levels. (F) Serum interleukin‐6 (IL‐6) levels. (G) Representative images of Myeloperoxidase (MPO) staining. (H) Quantitative of MPO staining. (I) Iron levels in the pancreas. (J and K) Representative images and relative fluorescence intensity of dihydroethidium (DHE) staining in the pancreas. (L) Malondialdehyde (MDA) levels in the pancreas. (M) GSH‐Px enzyme activity. (N) Ultrastructural alterations in the pancreas. (O and P) Western blot analysis of the PTGS2 and glutathione peroxidase (GPX4) expression level in the pancreas. (Q and R) Western blot analysis of the P62 and LC3B expression level in the pancreas. (S) Five‐day survival of L‐arginine‐AP mice. (T) Five‐day survival of cerulein + LPS‐AP mice. n = 4‒15, error bars indicate the SEM; * p < .05 versus WT‐AP; # p < .05 versus KO‐AP. Fer‐1, Ferrostatin‐1; L‐arg, L‐arginine; TEM, transmission electron microscopy; WT, wild type.
Article Snippet: Arginine‐induced AP models were established in mice via intraperitoneal injection of
Techniques: Inhibition, Knock-Out, Staining, Fluorescence, Activity Assay, Western Blot, Expressing, Transmission Assay, Electron Microscopy